Chromosome Analysis - Cytogenetic Tests
Fluorescence In Situ Hybridization (FISH) - Molecular Cytogenetic Tests
Molecular (DNA) Tests
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Fluorescence In Situ Hybridization (FISH)
Fluorescence In Situ Hybridization is used to identify specific chromosomal regions on cytologic preparations. FISH is generally performed on stimulated peripheral blood so that both chromosome analysis and the identification of specific translocations may be performed. FISH is recommended on unstimulated peripheral blood for minimal residual disease in the event that a patient has already had their chromosomes analyzed. FISH is also commonly used to identify specific chromosomal deletions associated with pediatric syndromes.
FISH, BCR-ABL, for translocation (9;22):
Chronic Myelogenous Leukemia and Acute Lymphocytic Leukemia. It is capable of detecting mosaicism as low as 2-3%.
FISH, IgH-BCL2, for translocation (14;18):
This test can be used to detect the translocation associated with B-cell Lymphoma. It is capable of detecting mosaicism as low as 2-3%.
FISH, RAR-APL, for translocation (15;17):
This test can be used to detect the translocation associated with Acute Promyelocytic Leukemia. It is capable of detecting mosaicism as low as 2-3%.
Molecular Cytogenetic Tests (FISH)
- FISH (fluor. in situ hybridization), stim. periph. Blood
- FISH, unstimulated peripheral blood
- FISH, BCR-ABL, for translocation (9;22)
- FISH, IgH-BCL2, for translocation (14;18)
- FISH, RAR-APL, for translocation (15;17)
- FISH, for minimal residual disease
- FISH, for microdeletion syndromes
Sample Requirements
|
Sample Requirements |
Preferred |
Minimum |
|
FISH, stimulated peripheral blood |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, unstimulated peripheral blood |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, BCR-ABL, for translocation (9;22) |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, IgH-BCL2, for translocation (14;18) |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, RAR-APL, for translocation (15;17) |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, for microdeletion syndromes |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
FISH, for minimal residual disease |
3-5 mLs Na heparin peripheral blood |
1 mL |
|
STABILITY OF SAMPLES FOR MOLECULAR CYTOGENETIC TESTING: 2 days @ room temperature |
|
